By Dr Desmond S. T. Nicholl
Des Nicholl offers a brand new, absolutely revised, and multiplied variation of his well known undergraduate-level textbook. The ebook keeps some of the positive factors of the unique variation and nonetheless bargains a concise technical advent to the topic of genetic engineering. it really is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and smooth purposes of genetic engineering. purposes lined within the ebook comprise genomics, protein engineering, gene treatment, cloning, transgenic animals and crops, and bioethics. An advent to Genetic Engineering is key analyzing for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Extra resources for An Introduction to Genetic Engineering (Studies in Biology)
The pellet can be dried and the nucleic acid resuspended in the buﬀer appropriate to the next stage of the experiment. 3 Radiolabelling of nucleic acids A major problem encountered in many cloning procedures is that of keeping track of the small amounts of nucleic acid involved. This problem is magniﬁed at each stage of the process, because losses mean that the amount of material usually diminishes after each step. One way of tracing the material is to label the nucleic acid with a radioactive molecule (usually a deoxynucleoside triphosphate (dNTP), labelled with 3H or 32P), so that portions of each reaction may be counted in a scintillation counter to determine the amount of nucleic acid present.
The operon is a cluster of genes that are related (often coding for enzymes in a metabolic pathway), and which are under the control of a single promoter/regulatory region. Perhaps the best known example of this arrangement is the lac operon (Fig. 6), which codes for the enzymes responsible for lactose catabolism. Within the operon there are three genes that code for proteins (termed structural genes) and an upstream control region encompassing the promoter and a regulatory site called the operator.
4. Restriction mapping. (a) The 15 kb fragment yields two fragments of 14 and 1 kb when cut with BamHI. (b) The EcoRI fragments of 12 and 3 kb can be orientated in two ways with respect to the BamHI site. The BamHI/EcoRI double digest gives fragments of 11, 3 and 1 kb, and therefore the relative positions of the BamHI and EcoRI sites are as shown in (c). Similar reasoning with the orientation of the 8 and 7 kb PstI fragments (d) gives the ﬁnal map (e). on RNA. These may be required for many of the stages in the preparation and analysis of recombinants, but as they are not directly involved in the construction of recombinant DNA molecules, they will not be described in detail.