By Wolfsberg Tyra G., Wetterstrand Kris A., Guyer Mark S., Collins Francis S., Baxevanis Andreas D.
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Extra info for A user's guide to the human genome
Examination of the alignment shows that the EST is missing 153 nt (nt 360–512 of the 34 mRNA), which corresponds to the fifth exon that is missing in BE798864. This gap is in frame, so the EST could encode a homologous yet shorter protein. Because of the nature of EST sequencing, ESTs often contain sequencing errors at a rate much higher than those of the finished or even draft genomic sequence. It is certainly encouraging that EST BE798864 aligns well with the genomic sequence and that its encoded protein could be in the same frame as that produced from the known gene.
8). The VAST program quite often reveals similarities between proteins that are not evident from simple BLAST or FASTA searches, so readers are encouraged to employ this and similar tools when trying to answer questions related to protein families. com/naturegenetics ture, type ‘SRY’ in the box and hit Go. Four three-dimensional structures are returned, one of which is 1HRY, the structure of the human SRY–DNA complex solved by NMR. Clicking on the 1HRY hyperlink takes the user to the Structure Summary page for 1HRY.
1). It is immediately clear that the 19 mapped ADAM genes are distributed among 11 chromosomes, and that some, such as those at the tips of the q arms of chromosomes 10 and 14, are close together. The list at the bottom of the page presents links to the 19 genes. Another way to search for homologous genes in the genome is through a basic local alignment search tool (BLAST) search at the NCBI or Ensembl. BLAT searches at UCSC are not as sensitive as BLAST searches and may not find as many homologous genes.